FACS (from my journal)
After 72 hours in the incubator, the cells are looked under the microscope for qualitative result by reflecting the certain wavelength to see how many cells glow. Then cells are prepared for FACS analysis by getting washed with DPBS, detached from the plate by trypsinization, and put into tubes.
72 hours after transfection, we put the transfected cells from the cell plates into microtubes to get the quantitative results from the FACS machine. It takes about 3 hours, and Tesa is going to teach me to solve the Rubik’s cube during every 3-5 min. break between changing tubes for the machine.
On Sunday, Ashley’s family is holding the annual cookout. Rigo and I are invited to stay at her house, and I’m so excited that I can’t stop thinking about it when I am working!
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